Biosynthesis of von Willebrand protein by human endothelial cells: processing steps and their intracellular localization.
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منابع مشابه
Biosynthesis of von Willebrand protein by human endothelial cells: processing steps and their intracellular localization
Biosynthesis of von Willebrand protein by human umbilical vein endothelial cells involved distinct processing steps marked by the presence of several intermediate molecular species. Examination of endoglycosidase H sensitivity of these intracellular intermediates indicated that the processing steps occurred in at least two separate cellular compartments. In the pre-Golgi apparatus (most probabl...
متن کاملBiosynthesis of von Willebrand Protein by Human Endothelial Cells" Processing and Their Intracellular Localization Steps
Biosynthesis of von Willebrand protein by human umbilical vein endothelial cells involved distinct processing steps marked by the presence of several intermediate molecular species. Examination of endoglycosidase H sensitivity of these intracellular intermediates indicated that the processing steps occurred in at least two separate cellular compartments. In the pre-Golgi apparatus (most probabl...
متن کاملBiosynthesis of von Willebrand protein by human megakaryocytes.
Immunofluorescence staining of buffy coat smears from a patient with chronic myelogenous leukemia in accelerated phase showed that approximately 13% of all nucleated cells contained von Willebrand protein and, therefore, appeared to be of megakaryocytic origin. This was confirmed by positive staining with antisera against platelet factor 4 and platelet glycoproteins. Short-term cultures of the ...
متن کاملSynthesis of von Willebrand factor by cultured human endothelial cells.
Cultured human endothelial cells synthesize and secrete a protein(s) which has Factor VIII antigen but which lacks Factor VIII clot-promoting activity (J. Clin. Invest. 52, 2757-2764, 1973). Von Willebrand factor activity has been identified in medium from cultured human endothelial cells. This activity was demonstrated by the ability to correct the defect in platelet adhesiveness of blood obta...
متن کاملMolecular Studies of von Willebrand Disease: Reduced von Willebrand Factor Biosynthesis, Storage, and Release in Endothelial Cells Derived From Patients
Endothelial cells were cultured from the umbilical veins of two neonates with type I von Willebrand disease (vWD) and compared with cells cultured in parallel from normal control umbilical veins. In both cases, cultured vWD endothelial cells contained less messenger RNA (mRNA) encoding von Willebrand factor (vWF), and constitutively secreted twoto fourfold less vWF protein than their matched co...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1984
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.99.6.2123